ABSTRACT
To determine the contents of salvianolic acid B and borneol in compound Danshen tablet (composed of Salviae Miltiorrhizae Radix et Rhizome, Notoginseng Radix et Rhizome and Borneolum Syntheticum) by near-infrared spectroscopy (NIR) and to establish a dependency model for rapid quantitative analysis. NIR data of 74 batches of compound Danshen tablet from different companies were collected; the contents of salvianolic acid B and borneol were determined by using high performanceliquid chromatography (HPLC) and gas chromatography (GC) respectively to establish the dependency model for salvianolic acid B and borneol. The results showed that the best waveband for salvianolic acid B was 10 846.2-10 013, 9 195.3-8 362.2, 6 719.1-4 242.8 cm⁻¹; root-mean-squares error of cross-validation (RMSECV), coefficient of determination ² and regression point displacement (RPD) of salvianolic acid B were 1.72 mg·g⁻¹, 91.05% and 7.93 mg·g⁻¹, respectively. While the best wavebands, RMSECV, ² and RPD of borneol were 10 846.2-5 060.5 cm⁻¹, 1.2 mg·g⁻¹, 96.11% and 6.71 mg·g⁻¹，respectively. The relative error of the established model as validation results for validation set samples was 2.67% and 4.64%， respectively.With the good predictability, the models can be applied to the real time monitoring and quality control of compound Danshen tablet.
ABSTRACT
Objective: To study the chemical constituents from the roots tubers of Serratula chinensis. Methods: The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties and spectral data. Results: Sixteen compounds (ecdysteroids) were isolated from the n-butanol fraction of 95% ethanol extract in the root tubers of S. chinensis, and their structures were identified as 20-hydroxyecdysone (1), polypodine B (2), carthamosterone (3), 20-hydroxyecdysone-20, 22-monoacetonide (4), 24-epi-abutasterone (5), polypodine C (6), coronatasterone (7), 20-hydroxyecdysone 2-O-β-D-glucopyranoside (8), 20-hydroxyecdysone 25-O-β-D-glucopyranoside (9), polypodine B 20, 22-acetonide (10), shidasterone (11), 2-O-acetyl-20-hydroxyecdysone (12), 3-O-acetyl-20-hydroxyecdysone (13), 20-hyderoxyecdysone-20, 22-butylidene acetal (14), 24-methylene-shidasterone (15), and ajugasterone D (16). Conclusion: Compounds 2, 4, 5, 7-10, 12, 15, and 16 are isolated from this plant for the first time, and compounds 5, 7-10, and 16 are found in the plants of genus Serratula L. for the first time.